COSMETICS – In vitro testing
- Cytotoxicity test (monolayer culture test).
- Irritation and sensitization (Elisa test)
- Eye irritation test on corneal epithelium
- 3D reconstituted (OECD TG492)
- Ocular corrosion test
- Skin irritation test on cells in monolayer
- Skin irritation test on in vitro reconstituted epidermis
- Skin corrosion test
- Percutaneous absorption test
- Skin sensitization test (THP-1, dendritic cells)
- Phototoxicity test
- Ames test: mutagenesis
- In vitro carcinogenesis test
- Antioxidant and antiradical activity test on skin cells (ROS analysis);
- Dermal anti-aging/redensifying activity test:
- Test of anti-inflammatory activity on monolayer cell cultures and reconstituted epidermis (3D tissues)
- Assessment of pro-inflammatory potential by analysis of cytokines and growth factors (IL-1α, IL-1, IL-8, GM-CSF, TNF-α)
- Cell proliferation assays on skin cells
- Melanogenesis modulation assay
- Percutaneous absorption test on the reconstituted epidermis
- Test of barrier effect on the reconstituted epidermis
- Test of wound healing activity on endothelial cells and keratinocytes/fibroblasts
- Testing modulation of immune response by analysis of specific mediators (example: histamine, IL-1α, TNF-α) for antipruritic activity, sensitization inhibition, immunostimulatory activity
- Assessment of cellular senescence
- Assay of seborestitutive activity: lipase inhibition
- Evaluation of 5-alpha-reductase inhibition
- In vitro moisturizing activity test
- SPF, UVA-PF, and criticality determination in vitro
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